Title: Development of a microbial consortium for production of blend of enzymes for hydrolysis of agricultural wastes into sugars
Authors: Vats, Siddharth; Maurya, Devendra Prasad; Shaimoon, Mohmed; Agarwal, Ayushi; Negi, Sangeeta
Abstract: This
study presents development of a blend of enzymes capable of degrading
lignocellulosic biomass from a locally isolated microbial consortium through
solid state fermentation. Different agro wastes (wheat bran, rice husk and pine
needles) were taken in all combinations as a substrate and at 1:1:1 ratio,
optimum production of blend of enzymes was obtained with 0.673 U/ml, 0.214 U/ml
and 0.032 U/ml activities respectively after 3 days of incubation at 37°C. This
blend of enzyme hydrolysed pine waste and released 0.454 g/g of sugar at 37°C
at 120 rpm for 48 h. Produced blend of enzymes was found very potent and can
have great application in biofuel, phytochemical and other industries.
Page(s): 585-590
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